This salamander is lungless like the four-towed and red-back salamanders.
The two-lined salamander has a broad yellowish-brown band down the back, and there may be numerous small, black spots within this stripe. Each side of the stripe is bordered with a black line. The sides of the body are dark grey and the belly
The two-lined salamander is found beneath logs or, more often, under rocks along the edge of streams.
The two-lined salamander can swim well and may be found in cool streams until April.
Once fertilized, the female deposits 20-50 eggs singly under rocks and logs beside running water. It may take several years before the larvae transform and move to the rocky areas at the edge of streams.
Very little is known about the diet of these salamanders.